ABSTRACT
Objective:To prepare vascular endothelial growth factor receptorⅡ ( VEGFRⅡ)-mediated targeted sulphur hexafluo-ride microbubble image agent and evaluate its targeting ability in human breast cancer MCF-7 in vitro. Methods:The sulphur hexafluo-ride microbubble image agent was prepared by a membrane dispersion-ultrasonic method and optimized by Box-Behnken design using design-expert 8. 0. 6 software. VEGFRⅡ-mediated targeted microbubble image agent was prepared by an absorption method. VEGFRⅡ-mediated targeted microbubble image agent was characterized by the morphology, particle size distribution and zeta potential. The targeting ability of the image agent in human breast cancer MCF-7 in vitro was detected by an immunofluorescent assay. Results:The particle size, polydispersion index and zeta potential of VEGFRⅡ-mediated targeted microbubble image agent was (3. 81 ± 0. 32) μm, 0. 261 ± 0. 037 and ( -25. 7 ± 2. 8) mV, respectively. The microbubbles were small and spherical with smooth surface as seen under a transmission electron microscope. VEGFRⅡ-mediated targeted microbubble image agent could combine with human breast cancer MCF-7 specifically in vitro. Conclusion: The absorption preparation technology of the targeted microbubble image agent is feasible, and the microbubble image agent has strong and especial targeting function in vitro.
ABSTRACT
Continuous pharmaceutical manufacturing is one of the development directions in international pharmaceutical technology. In this study, a continuous mixing technology of ethanol and concentrated extract in the ethanol precipitation of Salvia miltiorrhiza was realized by using a membrane dispersion method. The effects of ethanol flowrate, concentrated extract flowrate, and flowrate ratio on ethanol precipitation results were investigated. With the increase of the flowrates of ethanol and concentrated extract, retention rate of active phenolic acids components was increased, and the total solid removal rate was decreased. The purity of active components in supernatants was mainly affected by the ratio of ethanol flowrate and concentrated extract flowrate. The mixing efficiency of adding ethanol under continuous flow mixing mode in this study was comparable to that of industrial ethanol precipitation. Continuous adding ethanol by using a membrane dispersion mixer is a promising technology with many advantages such as easy enlargement, large production per unit volume, and easy control.